1,10-Phenanthroline hydrate 99% CAS NO.5144-89-8

1,10-Phenanthroline hydrate Basic information
Product Name:    1,10-Phenanthroline hydrate
Synonyms:    1,10-PHENANTHROLINE H2O;1,10-Phenanthroline monohydrate, reagent ACS;1,10-Phenanthrolinemonhydrate;1,10-Phenanthroline monohydrate, ACS;1,10-Phenanthroline, ACS, 99%;1,10-PHENANTHROLINE MONOHYDRATE extrapure AR;1,10-Phenanthroline, Monohydrate, Reagent;1,10-Phenanthroline Monohydrate [for Biochemical Research]
CAS:    5144-89-8
MF:    C12H8N2.H2O
MW:    198.22
EINECS:    627-114-9
Product Categories:    Miscellaneous;Analytical Chemistry;Chelating Reagents;Environmentally-friendly Oxidation;Ligands (Environmentally-friendly Oxidation);Phenanthrolines;Synthetic Organic Chemistry;ACS Grade;Analytical Reagents;Analytical/Chromatography;Bioactive Small Molecules;Building Blocks;Cell Biology;Chemical Synthesis;Essential Chemicals;Heterocyclic Building Blocks;Indicators;Inorganic Salts;N-Containing;Others;P;Redox Indicators;Research Essentials;Solutions and Reagents;Titration;bc0001;5144-89-8
Mol File:    5144-89-8.mol

1,10-Phenanthroline hydrate Chemical Properties
Melting point     100-104 °C(lit.)
density     1.10
vapor density     6.2 (vs air)
storage temp.     Sealed in dry,Room Temperature
solubility     methanol: soluble200MM (Stock solution is stable for months at –20° C.)
form     Needle-Like Crystals
color     Off-white, may become cream-colored during storage
Water Solubility     <0.01 g/100 mL at 21 ºC
Merck     14,7214
BRN     4008096
Stability:    Stable. Incompatible with iron, other heavy metals, strong oxidizing agents, strong acids.
CAS DataBase Reference    5144-89-8(CAS DataBase Reference)
EPA Substance Registry System    1,10-Phenanthroline, monohydrate (5144-89-8)

1,10-Phenanthroline hydrate Usage And Synthesis
Chemical Properties    white crystals or powder
Uses    1,10-Phenanthroline monohydrate is useful to study the DNA-protein interactions due to its nuclease activity. It is used as a redox indicator and reagent for spectrophotometric assay of silver. It acts as a chelating ligand for the determination of Fe, Pd and V. It also acts as a matrix metalloproteinase inhibitor. It is a photometric reagent for iron, which forms a red chelate with ferrous (iron II) ion.
Uses    When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.
1,10-Phenanthroline monohydrate is an aromatic nitrogen heterocycle that may be used as a chelating agent in the preparation of metal-chelate complexes.
General Description    White crystalline powder or solid. Slight odor.
Air & Water Reactions    Insoluble in water.
Reactivity Profile    1,10-Phenanthroline hydrate is incompatible with strong oxidizers. 1,10-Phenanthroline hydrate is also incompatible with strong acids. 1,10-Phenanthroline hydrate forms complex compounds with ferrous ions. .
Fire Hazard    Flash point data for 1,10-Phenanthroline hydrate are not available. 1,10-Phenanthroline hydrate is probably combustible.
Biochem/physiol Actions    Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.
Purification Methods    Crystallise its picrate (m 191o) from EtOH; then the free base is liberated with aqueous alkali, dried at 78o/8mm over P2O5 and crystallised from pet ether (b 80-100o). [Cumper et al. J Chem Soc 1188 1962.] It can be purified by zone melting. It has also been crystallised from hexane, *benzene/pet ether (b 40-60o) or sodium-dried *benzene, dried and stored over H2SO4. The monohydrate is obtained by crystallisation from aqueous EtOH or ethyl acetate. It has been crystallised from H2O (300 parts) to give the monohydrate m 102-103o which sublimes at 10-3mm [Fielding & LeFevre J Chem Soc 1811 1951.] The anhydrous compound has m 118o (after drying at high vacuum at 80o) and is also obtained by recrystallisation from pet ether or *C6H6 (70 parts) and drying at 78o/8mm. [UV: Badger et al. J Chem Soc 3199 1951.] IthasapKainH2Oof 4.857 (25o) or 5.02 (20o) and 4.27 in 50% aqueous EtOH (20o). [Albert et al. J Chem Soc 2240 1948]. [Beilstein 23 H 227, 23 II 235, 23/8 V 419.]